Journal: Cancers

Article Title: 5-Aminolevulinic Acid: A Novel Approach to Improving Radioresistance in Prostate Cancer.

doi: 10.3390/cancers17081286

Figure Lengend Snippet: Figure 4. (a) ABCG2 expression in radioresistant PCa cells treated with si-NC or si-ABCG2 was measured via Western blot analysis. Intracellular PpIX expression in the radioresistant PCa cells treated with si-NC or si-ABCG2 was measured using a microplate spectrophotometer ((b) PC-3-R; (c) DU 145-R). (d) Relative survival fractions of DU 145-R cells treated with si-NC or si-ABCG2 were compared among each group. 5-ALA: 5-aminolevulinic acid. ABCG2: ATP-binding cassette transporter subfamily G2. IR: irradiation. NC: negative control. ns: not significant. PCa: prostate cancer. PpIX: protoporphyrin IX. * p < 0.05, ** p < 0.01, **** p < 0.0001. Original western blots are presented in File S1.

Article Snippet: The primary antibodies used included anti-HIF-1a rabbit polyclonal antibody (cat. no. R12-2180; dilution 1:100; Assay Biotechnology Company, Fremont, CA, USA), anti-ABCG2 rabbit polyclonal antibody (cat. no. 27286-1-AP; dilution 1:100; Proteintech), anti-4-hydroxynonenal (4-HNE) rabbit polyclonal antibody (cat. no. ab46545; dilution 1:200; Abcam, Cambridge, UK), and anti-cleaved caspase 3 (C-caspase 3) rabbit monoclonal antibody (cat. no. 9664; dilution 1:200; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Western Blot, Spectrophotometry, Binding Assay, Irradiation, Negative Control

Journal: Cancers

Article Title: 5-Aminolevulinic Acid: A Novel Approach to Improving Radioresistance in Prostate Cancer.

doi: 10.3390/cancers17081286

Figure Lengend Snippet: Figure 5. (a) Survival curves of the relative survival fractions of Myc-CaP and Myc-CaP-R after IR (2–8 Gy single dose). (b) HIF-1a and ABCG2 expression was measured via Western blot analysis in Myc-CaP and Myc-CaP-R cells. (c) Photographs of the tumor region inoculated with Myc-CaP-R cells 7 days after treatment in animal experiments, along with (d) the actual tumor volumes at the time of euthanasia. (e–g) C-caspase 3 and 4-HNE expression in the four groups (normal control, 5-ALA alone,

Article Snippet: The primary antibodies used included anti-HIF-1a rabbit polyclonal antibody (cat. no. R12-2180; dilution 1:100; Assay Biotechnology Company, Fremont, CA, USA), anti-ABCG2 rabbit polyclonal antibody (cat. no. 27286-1-AP; dilution 1:100; Proteintech), anti-4-hydroxynonenal (4-HNE) rabbit polyclonal antibody (cat. no. ab46545; dilution 1:200; Abcam, Cambridge, UK), and anti-cleaved caspase 3 (C-caspase 3) rabbit monoclonal antibody (cat. no. 9664; dilution 1:200; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: 5-Aminolevulinic Acid: A Novel Approach to Improving Radioresistance in Prostate Cancer.

doi: 10.3390/cancers17081286

Figure Lengend Snippet: Figure 6. Immunohistochemical evaluation of (a,b) HIF-1a and (a,c) ABCG2 expression in human PCa specimens (pre-radiation and post-recurrence) was conducted. ABCG2: ATP-binding cassette transporter subfamily G2. HIF-1a: hypoxia-inducible factor 1a. PCa: prostate cancer. **** p < 0.0001.

Article Snippet: The primary antibodies used included anti-HIF-1a rabbit polyclonal antibody (cat. no. R12-2180; dilution 1:100; Assay Biotechnology Company, Fremont, CA, USA), anti-ABCG2 rabbit polyclonal antibody (cat. no. 27286-1-AP; dilution 1:100; Proteintech), anti-4-hydroxynonenal (4-HNE) rabbit polyclonal antibody (cat. no. ab46545; dilution 1:200; Abcam, Cambridge, UK), and anti-cleaved caspase 3 (C-caspase 3) rabbit monoclonal antibody (cat. no. 9664; dilution 1:200; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Expressing, Binding Assay

Journal: Exploration of Targeted Anti-tumor Therapy

Article Title: Hexachlorobenzene as a differential modulator of the conventional and metronomic chemotherapy response in triple negative breast cancer cells

doi: 10.37349/etat.2024.00218

Figure Lengend Snippet: Expression of ABCG2 in MDA-MB231 cells. (A) Expression of ABCG2 determined by western blot assays in cells treated with HCB (10 nmol/L), PX (100 mmol/L) or both, in the absence or presence of the NF-κB pathway inhibitor IMD (500 nmol/L); (B) expression of ABCG2 determined by western blot assays in cells treated with PX: 10 nmol/L + Carb (10 pmol/L), HCB (10 nmol/L) or both, in the absence or presence of IMD (500 nmol/L). Molecular weights are indicated on the right. Densitometric analysis of the bands is expressed as optical density units relative to the expression of GAPDH protein used as loading control. One representative experiment of three is shown. Values are the mean ± SEM of three experiments

Article Snippet: Samples (70 μg protein per lane) were subjected to 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis minigel electrophoresis, transferred to nitrocellulose membranes, and incubated overnight with rabbit anti-human ABCG2 polyclonal antibodies (Santa Cruz Biotechnology Inc., Dallas, Texas, USA) diluted 1:200.

Techniques: Expressing, Western Blot

Journal: The Korean Journal of Helicobacter and Upper Gastrointestinal Research

Article Title: Outcomes Associated With ABCG2 and CD133 Expression in Patients With Gastric Cancer After Surgical Resection

doi: 10.7704/kjhugr.2023.0039

Figure Lengend Snippet: Immunohistochemistry of ABCG2 and CD133 expression by grade (immunohistochemical staining, ×100). Tissue sections of 4-μm thick were incubated with rabbit polyclonal anti-ABCG2 monoclonal antibodies (dilution, 1:100, cat. no. sc-18841; Santa Cruz Biotechnology, Dallas, TX, USA) and anti-CD133 antibodies (dilution, 1:650, GTX60471; GeneTex, San Antonio, TX, USA). The sections were incubated with the anti-CD133 antibodies. Scale bars=200 μm. ABCG2, adenosine triphosphate-binding cassette subfamily G member 2.

Article Snippet: The sections were then incubated with rabbit polyclonal anti-ABCG2 monoclonal antibodies (dilution, 1:100, cat. no. sc-18841; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunohistochemistry, Expressing, Immunohistochemical staining, Staining, Incubation, Bioprocessing, Binding Assay

Journal: The Korean Journal of Helicobacter and Upper Gastrointestinal Research

Article Title: Outcomes Associated With ABCG2 and CD133 Expression in Patients With Gastric Cancer After Surgical Resection

doi: 10.7704/kjhugr.2023.0039

Figure Lengend Snippet: CD133 and ABCG2 expression by cancer stage. A: ABCG2 expression. B: CD133 expression. ABCG2, adenosine triphosphate- binding cassette subfamily G member 2.

Article Snippet: The sections were then incubated with rabbit polyclonal anti-ABCG2 monoclonal antibodies (dilution, 1:100, cat. no. sc-18841; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Binding Assay

Journal: The Korean Journal of Helicobacter and Upper Gastrointestinal Research

Article Title: Outcomes Associated With ABCG2 and CD133 Expression in Patients With Gastric Cancer After Surgical Resection

doi: 10.7704/kjhugr.2023.0039

Figure Lengend Snippet: Overall and recurrence-free survival by ABCG2 and CD133 expression. ABCG2, adenosine triphosphate-binding cassette subfamily G member 2.

Article Snippet: The sections were then incubated with rabbit polyclonal anti-ABCG2 monoclonal antibodies (dilution, 1:100, cat. no. sc-18841; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Binding Assay

Journal: The Korean Journal of Helicobacter and Upper Gastrointestinal Research

Article Title: Outcomes Associated With ABCG2 and CD133 Expression in Patients With Gastric Cancer After Surgical Resection

doi: 10.7704/kjhugr.2023.0039

Figure Lengend Snippet: Overall and recurrence-free survival by ABCG2-CD133 expression in four groups. A: Survival curve for four groups. B: Survival curve for two groups. ABCG2, adenosine triphosphate-binding cassette subfamily G member 2.

Article Snippet: The sections were then incubated with rabbit polyclonal anti-ABCG2 monoclonal antibodies (dilution, 1:100, cat. no. sc-18841; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Binding Assay

Journal: The Korean Journal of Helicobacter and Upper Gastrointestinal Research

Article Title: Outcomes Associated With ABCG2 and CD133 Expression in Patients With Gastric Cancer After Surgical Resection

doi: 10.7704/kjhugr.2023.0039

Figure Lengend Snippet: Adjusted curve of overall and recurrence-free survival rates. ABCG2, adenosine triphosphate-binding cassette subfamily G member 2.

Article Snippet: The sections were then incubated with rabbit polyclonal anti-ABCG2 monoclonal antibodies (dilution, 1:100, cat. no. sc-18841; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Binding Assay

Journal: The Korean Journal of Helicobacter and Upper Gastrointestinal Research

Article Title: Outcomes Associated With ABCG2 and CD133 Expression in Patients With Gastric Cancer After Surgical Resection

doi: 10.7704/kjhugr.2023.0039

Figure Lengend Snippet: Survival analysis based on ABCG2 expression and administration of systemic chemotherapy. ABCG2, adenosine triphosphate-binding cassette subfamily G member 2.

Article Snippet: The sections were then incubated with rabbit polyclonal anti-ABCG2 monoclonal antibodies (dilution, 1:100, cat. no. sc-18841; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Binding Assay